fugene hd (Promega)
90
Structured Review
Promega
fugene hd
Fugene Hd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fugene+hd/fugene+hd/pmc12221515-180-6-8
Average 90 stars, based on 1 article reviews
Fugene Hd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fugene+hd/fugene+hd/pmc12221515-180-6-8
Average 90 stars, based on 1 article reviews
fugene hd - by Bioz Stars,
2026-09
90/100 stars
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Generated:Article Title: A proximity-labeling-based approach to directly detect mRNA delivery to specific subcellular locations Article Snippet: HEK293T and A549 cells were maintained in high-glucose DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco). .. Lentiviral stocks were generated by transfection of HEK293T cells with the packaging vectors pMD2.9 (Addgene 12259) and psPAX2 (Addgene 12260), with either ERM-APEX2 (Addgene 79055) or APEX2-NES (Addgene 92158) included to target APEX2 to the endoplasmic reticulum or cytoplasm, respectively, using Transfection:Article Title: A proximity-labeling-based approach to directly detect mRNA delivery to specific subcellular locations Article Snippet: HEK293T and A549 cells were maintained in high-glucose DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco). .. Lentiviral stocks were generated by transfection of HEK293T cells with the packaging vectors pMD2.9 (Addgene 12259) and psPAX2 (Addgene 12260), with either ERM-APEX2 (Addgene 79055) or APEX2-NES (Addgene 92158) included to target APEX2 to the endoplasmic reticulum or cytoplasm, respectively, using Article Title: Compositions and methods for the diagnosis and treatment of lymphatic system disorders Article Snippet: The S214P mutation was introduced by site-directed mutagenesis using the Q5 mutagenesis kit from NEB following the manufacturer's instructions. .. Transfections in HEK293T and HeLa were performed using Article Title: Activation of bioluminescence by structural complementation Article Snippet: On day 1, a transfection mixture of 2 ng NLPoly156, NLPoly11S or NanoLuc® luciferase (Nluc) DNA, 1 ug pGEM3Zf(+) carrier DNA, 4 ul Fugene HD (Promega Corporation) and Phenol red-free OptiMEM to 100 ul was made and incubated at RT for 10 minutes. .. On day 1, a transfection mixture of 2 ng NLPoly156, NLPoly11S or NanoLuc® luciferase (Nluc) DNA, 1 ug pGEM3Zf(+) carrier DNA, 4 ul Article Title: Development of an inhalable dry powder formulation for inhibition of SARS-CoV-2 Article Snippet: .. Transfection: transfection mix was prepared using Article Title: Article Snippet: .. To generate stable cell line, HEK293 EBNA cells were transfected with the expression vector using Mutagenesis:Article Title: Compositions and methods for the diagnosis and treatment of lymphatic system disorders Article Snippet: The S214P mutation was introduced by site-directed mutagenesis using the Q5 mutagenesis kit from NEB following the manufacturer's instructions. .. Transfections in HEK293T and HeLa were performed using Luciferase:Article Title: Activation of bioluminescence by structural complementation Article Snippet: On day 1, a transfection mixture of 2 ng NLPoly156, NLPoly11S or NanoLuc® luciferase (Nluc) DNA, 1 ug pGEM3Zf(+) carrier DNA, 4 ul Fugene HD (Promega Corporation) and Phenol red-free OptiMEM to 100 ul was made and incubated at RT for 10 minutes. .. On day 1, a transfection mixture of 2 ng NLPoly156, NLPoly11S or NanoLuc® luciferase (Nluc) DNA, 1 ug pGEM3Zf(+) carrier DNA, 4 ul Incubation:Article Title: Activation of bioluminescence by structural complementation Article Snippet: On day 1, a transfection mixture of 2 ng NLPoly156, NLPoly11S or NanoLuc® luciferase (Nluc) DNA, 1 ug pGEM3Zf(+) carrier DNA, 4 ul Fugene HD (Promega Corporation) and Phenol red-free OptiMEM to 100 ul was made and incubated at RT for 10 minutes. .. On day 1, a transfection mixture of 2 ng NLPoly156, NLPoly11S or NanoLuc® luciferase (Nluc) DNA, 1 ug pGEM3Zf(+) carrier DNA, 4 ul Article Title: Development of an inhalable dry powder formulation for inhibition of SARS-CoV-2 Article Snippet: .. Transfection: transfection mix was prepared using Article Title: Activation of bioluminescence by structural complementation Article Snippet: 1 ug plasmid DNA containing a non-luminescent polypeptide mutant was mixed with OptiMEM (Life Technologies) to a final volume of 52 ul. .. 3.3 μl of Expressing:Article Title: Development of an inhalable dry powder formulation for inhibition of SARS-CoV-2 Article Snippet: .. Transfection: transfection mix was prepared using Article Title: Article Snippet: .. To generate stable cell line, HEK293 EBNA cells were transfected with the expression vector using Plasmid Preparation:Article Title: Development of an inhalable dry powder formulation for inhibition of SARS-CoV-2 Article Snippet: .. Transfection: transfection mix was prepared using Article Title: Article Snippet: .. To generate stable cell line, HEK293 EBNA cells were transfected with the expression vector using other:Article Title: Chimeric PD-1 receptor redirects primary T cells against childhood solid tumors but not to PD-1 ligand-positive CD80-coexpressing cells Article Snippet: To generate RD114-pseudotyped retroviruses, lipofection was used to transfect Transduction:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Media was replaced with fresh media supplemented with ViralBoost (Alstem, NC0966705) 6 hours post-transfection. .. Lentiviral Packaging and Transduction Lentiviral particles were produced by co-transfecting lentiviral transfer plasmids with standard packaging vectors psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) into HEK293T using Produced:Article Title: Compositions and methods for making epigenetic modifications Article Snippet: Media was replaced with fresh media supplemented with ViralBoost (Alstem, NC0966705) 6 hours post-transfection. .. Lentiviral Packaging and Transduction Lentiviral particles were produced by co-transfecting lentiviral transfer plasmids with standard packaging vectors psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) into HEK293T using Stable Transfection:Article Title: Article Snippet: .. To generate stable cell line, HEK293 EBNA cells were transfected with the expression vector using |